Wenping Gong
Institute for Tuberculosis Research, The 8th Medical Center of Chinese PLA General Hospital, China
Title: Evaluation of immunotherapeutic efficacy of vaccae vaccine and identification of differentially expressed genes in BALB/c mice infected with Mycobacterium tuberculosis
Biography
Biography: Wenping Gong
Abstract
Background: Tuberculosis (TB) is the leading cause from a single infectious agent and one of the top 10 causes of death. Mycobacterium vaccae vaccine is the first preventive TB vaccine in phase III trials besides BCG. However, the differentially expressed (DE) genes of host infected with M. tuberculosis before and after M. vaccae vaccine treatment is still poorly understood.
Methods: In the present study, the immunotherapeutic efficacy of M. vaccae vaccine were assessed in BALB/c mice by weight measurement, colony formation units counting, organ coefficient evaluation, and histopathology. The total RNA was extracted from isolated peripheral blood mononuclear cells from BALB/c mice, and the DE genes were identified by microarray, and GO analysis and pathway analysis were performed.
Results: M. vaccae vaccine had a significant immunotherapeutic effect in mouse model, and 2,326 upregulated and 2,221 downregulated genes were identified to be DE genes in M. vaccae group compared with control group. Additionally, a total of 123 signaling pathways with significant differences were identified, and our speculation suggested that the upregulated and downregulated pathways most related to M. vaccae might be MyD88-dependent TLR signaling pathway and PI3K-Akt signaling pathway.
Conclusion: Immunotherapeutic effect of M. vaccae might be enhanced via upregulating MyD88-dependent TLR signaling pathway and downregulating PI3K-Akt signaling pathway, which improved our understanding of the mechanism of M. vaccae vaccine immunotherapy.
Recent Publications
1. Gordon SV, Parish T. Microbe Profile: Mycobacterium tuberculosis: Humanity's deadly microbial foe. Microbiology (Reading, England) 2018;164(4):437-39 doi: 10.1099/mic.0.000601[published Online First: Epub Date]|.
2. Gong W, Liang Y, Wu X. The current status, challenges, and future developments of new tuberculosis vaccines. Hum Vaccin Immunother 2018;14(7):1697-716 doi: 10.1080/21645515.2018.1458806[published Online First: Epub Date]|.
3. Colditz GA, Brewer TF, Berkey CS, Wilson ME, Burdick E, Fineberg HV, Mosteller F. Efficacy of BCG vaccine in the prevention of tuberculosis. Meta-analysis of the published literature. Jama 1994;271(9):698-702
4. Fine PE. Variation in protection by BCG: implications of and for heterologous immunity. Lancet 1995;346(8986):1339-45
5. WHO. Global tuberculosis report 2017. Geneva: World Health Organization, 2017:1-262.5.